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  • Image Description: 3D confocal reconstruction of Bmi1+ lineage regenerative response in the jejunum at 7 d after 12 Gy irradiaton highlights confluent patches of Bmi1+-derived cells in multiple adjacent crypts/villi. Submitted by: (Yan, PNAS 2012) Kuo Lab, Stanford

Protocol Library

2015 - 2019 Archive
Protocols for Human Intestinal Stem Cell Work
A novel morphometry system automatically assessing the growth and regeneration of intestinal organoids (Wang - Third Military Medical University - China) - October/2018
Conditioned Medium Made from Secreted Media from Cultured Cell Lines Protocol (Estes - Baylor College of Medicine) - September/2015
Enteroid Maintenance Protocol (Estes - Baylor College of Medicine) - September/2015
Generating enteroids from Biopsy Samples Protocol (Estes - Baylor College of Medicine) - September/2015
Establishment of Human Enteroids (Helmrath – Cincinnati Children’s Hospital) - September/2015
Generation of Induced Pluripotent Stem Cells from Human Fibroblasts Using Lentivirus (Wells - Cincinnati Children’s Hospital) - October/2015
Generation of Induced Pluripotent Stem Cells from Human Fibroblasts Using Episomal Plasmids (Wells - Cincinnati Children’s Hospital) - October/2015
2D Culture of Human Enteroid Monolayers (Donowitz – Johns Hopkins Hospital) - September/2015
Human Crypt Isolation (Donowitz – Johns Hopkins Hospital) - September/2015
Comparison of Short vs. Long Method for Human Crypts Isolation (Martin – UCLA) - October/2015
Growing HB8 Myofibroblasts Protocol (Martin – UCLA) - October/2015
Human Intestinal Crypts for Enteroids (Martin – UCLA) - September/2015
Human Crypt Isolation and Propagation (Spence – University of Michigan) - October/2015
NIH Human Subject Information
Human Subject Infographic
          http://grants.nih.gov/grants/policy/hs/index.htm
Preparing the Human Subjects Section
          https://gds.nih.gov/
Protocols for Mouse Intestinal Stem Cell Work

Mouse GI Organoids (Helmrath – Cincinnati Children’s Hospital)
Intestinal Mesenchyme Isolation V1 (Li Lab- Stowers Institute)